blotting roller aluminum foil imaging system (Eppendorf AG)
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Eppendorf AG
blotting roller aluminum foil imaging system
Blotting Roller Aluminum Foil Imaging System, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 27337 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blotting+roller+aluminum+foil+imaging+system/Tubes/pmc13127243-82-282-290
Average 99 stars, based on 27337 article reviews
Blotting Roller Aluminum Foil Imaging System, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 27337 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blotting+roller+aluminum+foil+imaging+system/Tubes/pmc13127243-82-282-290
Average 99 stars, based on 27337 article reviews
blotting roller aluminum foil imaging system - by Bioz Stars,
2026-10
99/100 stars
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Molecular Weight:Article Title: Surface Plasmon Resonance (SPR) Workflow for Comparative Analysis of Nanobody Variants Binding to Lysozyme as a Model Ligand Article Snippet: Samples (see Basic Protocol ) 2× SDS buffer (Novex Tris‐Glycine SDS Sample Buffer (2×), Thermo Fisher Scientific, cat. no. LC2676, or equivalent) 1× Tris‐glycine SDS buffer (Novex Tris‐Glycine SDS Running Buffer, Thermo Fisher Scientific, cat. no. LC26754, or equivalent) Protein molecular‐weight marker (PageRuler Plus Prestained Protein Ladder, Thermo Fisher Scientific, cat. no. 26619, or equivalent) Coomassie blue stain (Imperial Protein Stain, Thermo Fisher Scientific, cat. no. 24617; PageBlue Protein Staining Solution, Thermo Fisher Scientific, cat. no. 24620; or similar reagent) Distilled water Transfer buffer (Pierce 1‐Step Transfer Buffer, Thermo Fisher Scientific, cat. no. 84731 × 5, or equivalent) Blocking buffer (Pierce Fast Blocking Buffer, Thermo Fisher Scientific, cat. no. 37575, or equivalent) Antibody solution: anti‐6×His‐Tag antibody solution (see recipe; make fresh) or Strep‐Tactin HRP solution (see recipe; make fresh) Wash buffer (Pierce Fast Wash Buffer, Thermo Fisher Scientific, cat. no. 37577, or equivalent) Chemiluminescent reagents (Thermo Scientific, cat. no. 34580, or equivalent) 50 mM TEAB/50% (v/v) ACN (see recipe; make fresh) 100% acetonitrile (ACN; Sigma‐Aldrich, cat. no. 34851) 100 mM TEAB (see recipe) 1 M DTT (see recipe) 1 M IAA (see recipe) 10 ng/μl trypsin (see recipe; make fresh) 10% (v/v) TFA (see recipe) 1% (v/v) TFA/50% (v/v) ACN (see recipe; make fresh) 0.1% (v/v) TFA/75% (v/v) ACN (see recipe; make fresh) 0.1% (v/v) formic acid (see recipe) 95°C water bath or dry bath Mini centrifuge Precast or custom‐made 4%‐20% protein gels (Novex Tris‐Glycine Mini Protein Gels, 4%‐20%, 1.0 mm, WedgeWell format, Invitrogen, cat. no. 15466694) Electrophoresis system, including gel box Nitrocellulose transfer stacks (Power Blotter Pre‐cut Membranes and Filters, nitrocellulose, Invitrogen, cat. no. PB7320) Black blot boxes or transparent trays Orbital shaker Semi‐dry transfer system (Invitrogen Power Blotter or equivalent) Marker:Article Title: Surface Plasmon Resonance (SPR) Workflow for Comparative Analysis of Nanobody Variants Binding to Lysozyme as a Model Ligand Article Snippet: Samples (see Basic Protocol ) 2× SDS buffer (Novex Tris‐Glycine SDS Sample Buffer (2×), Thermo Fisher Scientific, cat. no. LC2676, or equivalent) 1× Tris‐glycine SDS buffer (Novex Tris‐Glycine SDS Running Buffer, Thermo Fisher Scientific, cat. no. LC26754, or equivalent) Protein molecular‐weight marker (PageRuler Plus Prestained Protein Ladder, Thermo Fisher Scientific, cat. no. 26619, or equivalent) Coomassie blue stain (Imperial Protein Stain, Thermo Fisher Scientific, cat. no. 24617; PageBlue Protein Staining Solution, Thermo Fisher Scientific, cat. no. 24620; or similar reagent) Distilled water Transfer buffer (Pierce 1‐Step Transfer Buffer, Thermo Fisher Scientific, cat. no. 84731 × 5, or equivalent) Blocking buffer (Pierce Fast Blocking Buffer, Thermo Fisher Scientific, cat. no. 37575, or equivalent) Antibody solution: anti‐6×His‐Tag antibody solution (see recipe; make fresh) or Strep‐Tactin HRP solution (see recipe; make fresh) Wash buffer (Pierce Fast Wash Buffer, Thermo Fisher Scientific, cat. no. 37577, or equivalent) Chemiluminescent reagents (Thermo Scientific, cat. no. 34580, or equivalent) 50 mM TEAB/50% (v/v) ACN (see recipe; make fresh) 100% acetonitrile (ACN; Sigma‐Aldrich, cat. no. 34851) 100 mM TEAB (see recipe) 1 M DTT (see recipe) 1 M IAA (see recipe) 10 ng/μl trypsin (see recipe; make fresh) 10% (v/v) TFA (see recipe) 1% (v/v) TFA/50% (v/v) ACN (see recipe; make fresh) 0.1% (v/v) TFA/75% (v/v) ACN (see recipe; make fresh) 0.1% (v/v) formic acid (see recipe) 95°C water bath or dry bath Mini centrifuge Precast or custom‐made 4%‐20% protein gels (Novex Tris‐Glycine Mini Protein Gels, 4%‐20%, 1.0 mm, WedgeWell format, Invitrogen, cat. no. 15466694) Electrophoresis system, including gel box Nitrocellulose transfer stacks (Power Blotter Pre‐cut Membranes and Filters, nitrocellulose, Invitrogen, cat. no. PB7320) Black blot boxes or transparent trays Orbital shaker Semi‐dry transfer system (Invitrogen Power Blotter or equivalent) Staining:Article Title: Surface Plasmon Resonance (SPR) Workflow for Comparative Analysis of Nanobody Variants Binding to Lysozyme as a Model Ligand Article Snippet: Samples (see Basic Protocol ) 2× SDS buffer (Novex Tris‐Glycine SDS Sample Buffer (2×), Thermo Fisher Scientific, cat. no. LC2676, or equivalent) 1× Tris‐glycine SDS buffer (Novex Tris‐Glycine SDS Running Buffer, Thermo Fisher Scientific, cat. no. LC26754, or equivalent) Protein molecular‐weight marker (PageRuler Plus Prestained Protein Ladder, Thermo Fisher Scientific, cat. no. 26619, or equivalent) Coomassie blue stain (Imperial Protein Stain, Thermo Fisher Scientific, cat. no. 24617; PageBlue Protein Staining Solution, Thermo Fisher Scientific, cat. no. 24620; or similar reagent) Distilled water Transfer buffer (Pierce 1‐Step Transfer Buffer, Thermo Fisher Scientific, cat. no. 84731 × 5, or equivalent) Blocking buffer (Pierce Fast Blocking Buffer, Thermo Fisher Scientific, cat. no. 37575, or equivalent) Antibody solution: anti‐6×His‐Tag antibody solution (see recipe; make fresh) or Strep‐Tactin HRP solution (see recipe; make fresh) Wash buffer (Pierce Fast Wash Buffer, Thermo Fisher Scientific, cat. no. 37577, or equivalent) Chemiluminescent reagents (Thermo Scientific, cat. no. 34580, or equivalent) 50 mM TEAB/50% (v/v) ACN (see recipe; make fresh) 100% acetonitrile (ACN; Sigma‐Aldrich, cat. no. 34851) 100 mM TEAB (see recipe) 1 M DTT (see recipe) 1 M IAA (see recipe) 10 ng/μl trypsin (see recipe; make fresh) 10% (v/v) TFA (see recipe) 1% (v/v) TFA/50% (v/v) ACN (see recipe; make fresh) 0.1% (v/v) TFA/75% (v/v) ACN (see recipe; make fresh) 0.1% (v/v) formic acid (see recipe) 95°C water bath or dry bath Mini centrifuge Precast or custom‐made 4%‐20% protein gels (Novex Tris‐Glycine Mini Protein Gels, 4%‐20%, 1.0 mm, WedgeWell format, Invitrogen, cat. no. 15466694) Electrophoresis system, including gel box Nitrocellulose transfer stacks (Power Blotter Pre‐cut Membranes and Filters, nitrocellulose, Invitrogen, cat. no. PB7320) Black blot boxes or transparent trays Orbital shaker Semi‐dry transfer system (Invitrogen Power Blotter or equivalent) Blocking Assay:Article Title: Surface Plasmon Resonance (SPR) Workflow for Comparative Analysis of Nanobody Variants Binding to Lysozyme as a Model Ligand Article Snippet: Samples (see Basic Protocol ) 2× SDS buffer (Novex Tris‐Glycine SDS Sample Buffer (2×), Thermo Fisher Scientific, cat. no. LC2676, or equivalent) 1× Tris‐glycine SDS buffer (Novex Tris‐Glycine SDS Running Buffer, Thermo Fisher Scientific, cat. no. LC26754, or equivalent) Protein molecular‐weight marker (PageRuler Plus Prestained Protein Ladder, Thermo Fisher Scientific, cat. no. 26619, or equivalent) Coomassie blue stain (Imperial Protein Stain, Thermo Fisher Scientific, cat. no. 24617; PageBlue Protein Staining Solution, Thermo Fisher Scientific, cat. no. 24620; or similar reagent) Distilled water Transfer buffer (Pierce 1‐Step Transfer Buffer, Thermo Fisher Scientific, cat. no. 84731 × 5, or equivalent) Blocking buffer (Pierce Fast Blocking Buffer, Thermo Fisher Scientific, cat. no. 37575, or equivalent) Antibody solution: anti‐6×His‐Tag antibody solution (see recipe; make fresh) or Strep‐Tactin HRP solution (see recipe; make fresh) Wash buffer (Pierce Fast Wash Buffer, Thermo Fisher Scientific, cat. no. 37577, or equivalent) Chemiluminescent reagents (Thermo Scientific, cat. no. 34580, or equivalent) 50 mM TEAB/50% (v/v) ACN (see recipe; make fresh) 100% acetonitrile (ACN; Sigma‐Aldrich, cat. no. 34851) 100 mM TEAB (see recipe) 1 M DTT (see recipe) 1 M IAA (see recipe) 10 ng/μl trypsin (see recipe; make fresh) 10% (v/v) TFA (see recipe) 1% (v/v) TFA/50% (v/v) ACN (see recipe; make fresh) 0.1% (v/v) TFA/75% (v/v) ACN (see recipe; make fresh) 0.1% (v/v) formic acid (see recipe) 95°C water bath or dry bath Mini centrifuge Precast or custom‐made 4%‐20% protein gels (Novex Tris‐Glycine Mini Protein Gels, 4%‐20%, 1.0 mm, WedgeWell format, Invitrogen, cat. no. 15466694) Electrophoresis system, including gel box Nitrocellulose transfer stacks (Power Blotter Pre‐cut Membranes and Filters, nitrocellulose, Invitrogen, cat. no. PB7320) Black blot boxes or transparent trays Orbital shaker Semi‐dry transfer system (Invitrogen Power Blotter or equivalent) Electrophoresis:Article Title: Surface Plasmon Resonance (SPR) Workflow for Comparative Analysis of Nanobody Variants Binding to Lysozyme as a Model Ligand Article Snippet: Samples (see Basic Protocol ) 2× SDS buffer (Novex Tris‐Glycine SDS Sample Buffer (2×), Thermo Fisher Scientific, cat. no. LC2676, or equivalent) 1× Tris‐glycine SDS buffer (Novex Tris‐Glycine SDS Running Buffer, Thermo Fisher Scientific, cat. no. LC26754, or equivalent) Protein molecular‐weight marker (PageRuler Plus Prestained Protein Ladder, Thermo Fisher Scientific, cat. no. 26619, or equivalent) Coomassie blue stain (Imperial Protein Stain, Thermo Fisher Scientific, cat. no. 24617; PageBlue Protein Staining Solution, Thermo Fisher Scientific, cat. no. 24620; or similar reagent) Distilled water Transfer buffer (Pierce 1‐Step Transfer Buffer, Thermo Fisher Scientific, cat. no. 84731 × 5, or equivalent) Blocking buffer (Pierce Fast Blocking Buffer, Thermo Fisher Scientific, cat. no. 37575, or equivalent) Antibody solution: anti‐6×His‐Tag antibody solution (see recipe; make fresh) or Strep‐Tactin HRP solution (see recipe; make fresh) Wash buffer (Pierce Fast Wash Buffer, Thermo Fisher Scientific, cat. no. 37577, or equivalent) Chemiluminescent reagents (Thermo Scientific, cat. no. 34580, or equivalent) 50 mM TEAB/50% (v/v) ACN (see recipe; make fresh) 100% acetonitrile (ACN; Sigma‐Aldrich, cat. no. 34851) 100 mM TEAB (see recipe) 1 M DTT (see recipe) 1 M IAA (see recipe) 10 ng/μl trypsin (see recipe; make fresh) 10% (v/v) TFA (see recipe) 1% (v/v) TFA/50% (v/v) ACN (see recipe; make fresh) 0.1% (v/v) TFA/75% (v/v) ACN (see recipe; make fresh) 0.1% (v/v) formic acid (see recipe) 95°C water bath or dry bath Mini centrifuge Precast or custom‐made 4%‐20% protein gels (Novex Tris‐Glycine Mini Protein Gels, 4%‐20%, 1.0 mm, WedgeWell format, Invitrogen, cat. no. 15466694) Electrophoresis system, including gel box Nitrocellulose transfer stacks (Power Blotter Pre‐cut Membranes and Filters, nitrocellulose, Invitrogen, cat. no. PB7320) Black blot boxes or transparent trays Orbital shaker Semi‐dry transfer system (Invitrogen Power Blotter or equivalent) Imaging:Article Title: Surface Plasmon Resonance (SPR) Workflow for Comparative Analysis of Nanobody Variants Binding to Lysozyme as a Model Ligand Article Snippet: Samples (see Basic Protocol ) 2× SDS buffer (Novex Tris‐Glycine SDS Sample Buffer (2×), Thermo Fisher Scientific, cat. no. LC2676, or equivalent) 1× Tris‐glycine SDS buffer (Novex Tris‐Glycine SDS Running Buffer, Thermo Fisher Scientific, cat. no. LC26754, or equivalent) Protein molecular‐weight marker (PageRuler Plus Prestained Protein Ladder, Thermo Fisher Scientific, cat. no. 26619, or equivalent) Coomassie blue stain (Imperial Protein Stain, Thermo Fisher Scientific, cat. no. 24617; PageBlue Protein Staining Solution, Thermo Fisher Scientific, cat. no. 24620; or similar reagent) Distilled water Transfer buffer (Pierce 1‐Step Transfer Buffer, Thermo Fisher Scientific, cat. no. 84731 × 5, or equivalent) Blocking buffer (Pierce Fast Blocking Buffer, Thermo Fisher Scientific, cat. no. 37575, or equivalent) Antibody solution: anti‐6×His‐Tag antibody solution (see recipe; make fresh) or Strep‐Tactin HRP solution (see recipe; make fresh) Wash buffer (Pierce Fast Wash Buffer, Thermo Fisher Scientific, cat. no. 37577, or equivalent) Chemiluminescent reagents (Thermo Scientific, cat. no. 34580, or equivalent) 50 mM TEAB/50% (v/v) ACN (see recipe; make fresh) 100% acetonitrile (ACN; Sigma‐Aldrich, cat. no. 34851) 100 mM TEAB (see recipe) 1 M DTT (see recipe) 1 M IAA (see recipe) 10 ng/μl trypsin (see recipe; make fresh) 10% (v/v) TFA (see recipe) 1% (v/v) TFA/50% (v/v) ACN (see recipe; make fresh) 0.1% (v/v) TFA/75% (v/v) ACN (see recipe; make fresh) 0.1% (v/v) formic acid (see recipe) 95°C water bath or dry bath Mini centrifuge Precast or custom‐made 4%‐20% protein gels (Novex Tris‐Glycine Mini Protein Gels, 4%‐20%, 1.0 mm, WedgeWell format, Invitrogen, cat. no. 15466694) Electrophoresis system, including gel box Nitrocellulose transfer stacks (Power Blotter Pre‐cut Membranes and Filters, nitrocellulose, Invitrogen, cat. no. PB7320) Black blot boxes or transparent trays Orbital shaker Semi‐dry transfer system (Invitrogen Power Blotter or equivalent) Software:Article Title: Surface Plasmon Resonance (SPR) Workflow for Comparative Analysis of Nanobody Variants Binding to Lysozyme as a Model Ligand Article Snippet: Samples (see Basic Protocol ) 2× SDS buffer (Novex Tris‐Glycine SDS Sample Buffer (2×), Thermo Fisher Scientific, cat. no. LC2676, or equivalent) 1× Tris‐glycine SDS buffer (Novex Tris‐Glycine SDS Running Buffer, Thermo Fisher Scientific, cat. no. LC26754, or equivalent) Protein molecular‐weight marker (PageRuler Plus Prestained Protein Ladder, Thermo Fisher Scientific, cat. no. 26619, or equivalent) Coomassie blue stain (Imperial Protein Stain, Thermo Fisher Scientific, cat. no. 24617; PageBlue Protein Staining Solution, Thermo Fisher Scientific, cat. no. 24620; or similar reagent) Distilled water Transfer buffer (Pierce 1‐Step Transfer Buffer, Thermo Fisher Scientific, cat. no. 84731 × 5, or equivalent) Blocking buffer (Pierce Fast Blocking Buffer, Thermo Fisher Scientific, cat. no. 37575, or equivalent) Antibody solution: anti‐6×His‐Tag antibody solution (see recipe; make fresh) or Strep‐Tactin HRP solution (see recipe; make fresh) Wash buffer (Pierce Fast Wash Buffer, Thermo Fisher Scientific, cat. no. 37577, or equivalent) Chemiluminescent reagents (Thermo Scientific, cat. no. 34580, or equivalent) 50 mM TEAB/50% (v/v) ACN (see recipe; make fresh) 100% acetonitrile (ACN; Sigma‐Aldrich, cat. no. 34851) 100 mM TEAB (see recipe) 1 M DTT (see recipe) 1 M IAA (see recipe) 10 ng/μl trypsin (see recipe; make fresh) 10% (v/v) TFA (see recipe) 1% (v/v) TFA/50% (v/v) ACN (see recipe; make fresh) 0.1% (v/v) TFA/75% (v/v) ACN (see recipe; make fresh) 0.1% (v/v) formic acid (see recipe) 95°C water bath or dry bath Mini centrifuge Precast or custom‐made 4%‐20% protein gels (Novex Tris‐Glycine Mini Protein Gels, 4%‐20%, 1.0 mm, WedgeWell format, Invitrogen, cat. no. 15466694) Electrophoresis system, including gel box Nitrocellulose transfer stacks (Power Blotter Pre‐cut Membranes and Filters, nitrocellulose, Invitrogen, cat. no. PB7320) Black blot boxes or transparent trays Orbital shaker Semi‐dry transfer system (Invitrogen Power Blotter or equivalent) Mass Spectrometry:Article Title: Surface Plasmon Resonance (SPR) Workflow for Comparative Analysis of Nanobody Variants Binding to Lysozyme as a Model Ligand Article Snippet: Samples (see Basic Protocol ) 2× SDS buffer (Novex Tris‐Glycine SDS Sample Buffer (2×), Thermo Fisher Scientific, cat. no. LC2676, or equivalent) 1× Tris‐glycine SDS buffer (Novex Tris‐Glycine SDS Running Buffer, Thermo Fisher Scientific, cat. no. LC26754, or equivalent) Protein molecular‐weight marker (PageRuler Plus Prestained Protein Ladder, Thermo Fisher Scientific, cat. no. 26619, or equivalent) Coomassie blue stain (Imperial Protein Stain, Thermo Fisher Scientific, cat. no. 24617; PageBlue Protein Staining Solution, Thermo Fisher Scientific, cat. no. 24620; or similar reagent) Distilled water Transfer buffer (Pierce 1‐Step Transfer Buffer, Thermo Fisher Scientific, cat. no. 84731 × 5, or equivalent) Blocking buffer (Pierce Fast Blocking Buffer, Thermo Fisher Scientific, cat. no. 37575, or equivalent) Antibody solution: anti‐6×His‐Tag antibody solution (see recipe; make fresh) or Strep‐Tactin HRP solution (see recipe; make fresh) Wash buffer (Pierce Fast Wash Buffer, Thermo Fisher Scientific, cat. no. 37577, or equivalent) Chemiluminescent reagents (Thermo Scientific, cat. no. 34580, or equivalent) 50 mM TEAB/50% (v/v) ACN (see recipe; make fresh) 100% acetonitrile (ACN; Sigma‐Aldrich, cat. no. 34851) 100 mM TEAB (see recipe) 1 M DTT (see recipe) 1 M IAA (see recipe) 10 ng/μl trypsin (see recipe; make fresh) 10% (v/v) TFA (see recipe) 1% (v/v) TFA/50% (v/v) ACN (see recipe; make fresh) 0.1% (v/v) TFA/75% (v/v) ACN (see recipe; make fresh) 0.1% (v/v) formic acid (see recipe) 95°C water bath or dry bath Mini centrifuge Precast or custom‐made 4%‐20% protein gels (Novex Tris‐Glycine Mini Protein Gels, 4%‐20%, 1.0 mm, WedgeWell format, Invitrogen, cat. no. 15466694) Electrophoresis system, including gel box Nitrocellulose transfer stacks (Power Blotter Pre‐cut Membranes and Filters, nitrocellulose, Invitrogen, cat. no. PB7320) Black blot boxes or transparent trays Orbital shaker Semi‐dry transfer system (Invitrogen Power Blotter or equivalent) |